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Finally, the stained cells were observed and photographed under a microscope at 100× magnification

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In addition, GPx-4 depletion in cell culture also promotes cell death by apoptotic mechanisms that could not be alleviated with water-soluble antioxidants, confirming that intracellularly GPx-4 mainly prevents lipid peroxidation, essentially antagonizing the actions of lipoxygenases and cycloogenase